An Immunoassay on Serum Human Growth Hormone

Immunoassay for Human Growth Hormone

Since highly purified human pituitary growth hormone has become available, much information concerning its metabolic effects has been accumulated. However, simple and specific methods of measuring endogenous levels of human growth hormone have been difficult to achieve. Attempts to assay human growth hormone by injecting whole serum into hypophysectomized rats and measuring the width of the tib...

Immunoassay of Serum Growth Hormone in Acromegalic Patients.

Immunoassay for human serum hepcidin.

We developed and validated the first serum enzyme-linked immunosorbent assay for hepcidin, the principal iron-regulatory hormone that has been very difficult to measure. In healthy volunteers, the 5% to 95% range of hepcidin concentrations was 29 to 254 ng/mL in men (n = 65) and 17 to 286 ng/mL in women (n = 49), with median concentrations 112 versus 65 (P < .001). The lower limit of detection ...

Immunoassay for human serum hemojuvelin.

BACKGROUND Hemojuvelin, a critical regulator of iron homeostasis, is involved in the regulation of hepcidin expression and iron homeostasis. It is expressed both as a membrane-bound form and as a soluble one. Serum hemojuvelin can be produced by secretion following furin cleavage or by proteolytic cleavage of the membrane-bound form by matriptase 2 (TMPRSS6). These forms contribute to down-regu...

An immunoassay for human serum hepcidin at last: Ganz klar?

The WASP gene is located on the X chromosome. Random X chromosome inactivation in WASp ϩ/-mice would theoretically result in 50% of cells expressing WASp. Westerberg et al show this to be the case in the myeloid compartment , that is, neutrophils, dendritic cells, macro-phages, and NK cells. Also, both papers show that in the most immature subsets of T and B cells, such as the double-negative o...